|
KMID : 0545120230330010051
|
|
Journal of Microbiology and Biotechnology
2023 Volume.33 No. 1 p.51 ~ p.60
|
|
|
Exploring the Microbial Community and Functional Characteristics of the Livestock Feces Using the Whole Metagenome Shotgun Sequencing
|
|
Kim Hye-Ri
Kim Eun-Sol
Cho Jin-Ho
Song Min-Ho
Cho Jae-Hyoung
Kim Sheena
Keum Gi-Beom
Kwak Jin-Ok
Doo Hyun-Ok
Pandey Sriniwas
Park Seung-Hwan
Lee Ju-Huck
Jung Hyun-Jung
Hur Tai-Young
Kim Jae-Kyung
Oh Kwang-Kyo
Kim Hyeun-Bum
Lee Ju-Hoon
|
|
|
Abstract
|
|
|
|
The foodborne illness is the important public health concerns, and the livestock feces are known to be one of the major reservoirs of foodborne pathogens. Also, it was reported that 45.5% of foodborne illness outbreaks have been associated with the animal products contaminated with the livestock feces. In addition, it has been known that the persistence of a pathogens depends on many potential virulent factors including the various virulent genes. Therefore, the first step to understanding the public health risk of livestock feces is to identify and describe microbial communities and potential virulent genes that contribute to bacterial pathogenicity. We used the whole metagenome shotgun sequencing to evaluate the prevalence of foodborne pathogens and to characterize the virulence associated genes in pig and chicken feces. Our data showed that the relative abundance of potential foodborne pathogens, such as Bacillus cereus was higher in chickens than pigs at the species level while the relative abundance of foodborne pathogens including Campylobacter coli was only detected in pigs. Also, the microbial functional characteristics of livestock feces revealed that the gene families related to “Biofilm formation and quorum sensing” were highly enriched in pigs than chicken. Moreover, the variety of gene families associated with “Resistance to antibiotics and toxic compounds” were detected in both animals. These results will help us to prepare the scientific action plans to improve awareness and understanding of the public health risks of livestock feces.
|
|
|
KEYWORD
|
|
|
Foodborne pathogens, livestock, livestock environment, metagenome shotgun sequencing, potential virulence
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|